High-fidelity sequencing methods

Match the following high-fidelity sequencing methods with their respective description Sample DNAIS amplified via rolling circle amplification which produces a longer strand of DNA containing multiple concatenated copies of the original DNA fragment. By sequencing the resulting strand, true mutations will appear in each copy of the original fragment. while sequencing errors will appear sporadically Barcodes are attached to both strands of a DNA fragment, which is then amplified. The two barcodes can be used to orient the forward strand and the reverse strand. If a mutation appears in the same position on both strands, it is likely real Otherwise, it is likely a sequencing error. 1. UMI redundant sequencing 2. Circle sequencing 3.

Double-stranded (Duplex) sequencing 4. Hybrid capture Instead of sequencing an entire DNA sample, special probes are used to enrich the proportion of certain regions of DNA which is then sequenced. This allows for much higher sequencing depth of regions of interest Barcodes are attached to both strands of a DNA fragment, which is then amplified. The two barcodes can be used to orient the forward strand and the reverse strand. If a mutation appears in the same position on both strands, it is likely real. Otherwise, it is likely a sequencing error. 1. UMI redundant sequencing 2. Circle sequencing 3. Double-stranded (Duplex) sequencing 4. Hybrid capture Instead of sequencing an entire DNA sample, special probes are used to enrich the proportion of certain regions of DNA, which is then sequenced. This allows for a much higher sequencing depth of regions of interest. Primers with unique DNA barcodes are used to amplify sample DNA, which is then sequenced. The barcode is then used to identify copies of the same DNA fragment. True mutations will show up in basically all sequences of the same fragment, while amplification or sequencing errors appear sporadically